関係者各位
沿岸環境科学研究センター
化学汚染・沿岸環境研究拠点（LaMer）
拠点長 岩田 久人
沿岸環境科学研究センター（CMES）、共同利用・共同研究拠点「化学汚染・沿岸環境研究拠点（LaMer）」プロジェクトの一環として、下記の要領で第26回特別講演会を行うこととなりました。言語は英語となります。聴講は無料・事前申し込み不要です。多数のご参加をお待ちしております。
26th LaMer Special Seminar “IMPACT OF FISH FARMING ON ANTIBIOTIC RESISTOME AND MOBILE GENETIC ELEMENTS IN BALTIC SEA SEDIMENT” is going to be held on December 3rd, 2018. Admission free, reservations are not required.
記
日時：2018年12月3日（月） 16：00 ～ 17：00
December 3rd, 2018
場所：総合研究棟2、2階演習室、#212
Seminar room #212, Science Research Bldg 2, CMES, Ehime University
演題：IMPACT OF FISH FARMING ON ANTIBIOTIC RESISTOME AND MOBILE GENETIC ELEMENTS IN
BALTIC SEA SEDIMENT
バルト海堆積物中の薬剤耐性遺伝子と可動性遺伝子伝達因子の動態に及ぼす魚類養殖の影響
講師：Dr. Windi Muziasari
Researcher in the University of Helsinki, and CEO of Resistomap Co.
趣旨：Antibiotic resistance has become a serious threat to the efficacy of antibiotics used in human and veterinary medicine. Understanding the abundance and prevalence of antibiotic resistance genes (ARGs) in the environmental resistome is important for maintaining the efficacy of antibiotics and predicting a risk of the ARGs spreading in the environment and moving into previously non-resistant bacteria, including human pathogens. Fish farms are an environmental reservoir of ARGs due to the treatment of fish with antibiotics that also are important for human medicine. The two main topics of the talk are (1) determining the abundance and diversity of ARGs and mobile genetic elements in sediments impacted by fish farming and (2) investigating the major source of ARGs in the farm sediments in the Northern Baltic Sea. In addition, correlations between ARGs and mobile genetic elements were examined to estimate the potential risk of ARG mobilization in the environment. This study employed a high-throughput SmartChip qPCR, which permits quantifying up to 384 ARGs and genes associated with mobile elements in the environmental resistome in a single experiment. Fish farming impacts the composition of ARGs in sediments below fish farms in the Northern Baltic Sea. However, the impact is local and mostly limited to enrichment of ARGs associated with antibiotics used at the farms. In the current conditions, the risk of ARG spread from the farm sediments to the surrounding sediments is low in the Northern Baltic Sea. However, the enriched ARGs persist in the farm sediments during the 6-year observations even when the selection pressure of the antibiotics is negligible. Moreover, significant correlations between mobile elements and ARGs may imply the persistence of certain ARGs in the fish farming environments and their potential for mobilizing the ARGs to other bacteria including pathogens. The persistence of ARGs at the farm facilities is a threat to the efficacy of t he antibiotics against fish diseases, potentially leading to fish production losses. We provide indirect evidence suggesting that certain ARGs are being constantly introduced by feces of the farmed fish into the sediments below the fish farms. Further studies could focus on investigating the development of ARGs in juvenile fish before they are introduced into the Baltic Sea open-cage farms. We conclude that the SmartChip qPCR is a powerful tool that provides unprecedented insights into the ARG composition in the environmental resistome associated with fish farming.
以上
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化学汚染・沿岸環境研究拠点
（Leading Academia in Marine and Environment Pollution Research）
Email: lamer@stu.ehime-u.ac.jp