関係者各位
沿岸環境科学研究センター
化学汚染・沿岸環境研究拠点（LaMer）
拠点長 岩田 久人
沿岸環境科学研究センター（ＣＭＥＳ）、共同利用・共同研究拠点「化学汚染・沿岸環境研究拠点（ＬａＭｅｒ）」プロジェクトの一環として、下記の要領で第２８回特別講演会を行うこととなりました。
聴講は無料・事前申し込み不要です。多くの皆様のご参加をお待ちしております。
記
【日 時】 ２０１９年５月３１日（金） １０：００～
【場 所】 総合研究棟１, ６階会議室（愛媛大学理学部キャンパス内）
【演 題】 １）Developmental neurobehavioral toxicity by non-coplanar PCB and
PBDE in zebrafish
ゼブラフィッシュにおけるPCBおよびPBDEによる発達行動神経毒性
２）Cytochrome P450 in cat : structure, expression, enzymatic
activity and polymorphism
ネコのシトクロムP450：構造、発現、酵素活性および多型
【講 師】 寺岡宏樹（Hiroki Teraoka）
酪農学園大学獣医学群 教授
（School of Veterinary Medicine, Rakuno Gakuen University）
【要 約】
Contamination with polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in the environment is still a major concern due to their persistent bioaccumulative toxicity that can disturb neurobehavioral functions especially by perinatal exposure. We studied developmental neurobehavioral toxicity by a non-coplanar PCB-dominant mixture (Aroclor 1254: ncPCB) and that of PBDE (BDE-47), using zebrafish embryos in vivo. Both organohalogens (OHs) increased tail shaking and rotation of embryos in a concentration-dependent manner. Chemical inhibition, knock-down and knock-out of tyrosinase, a rate limiting enzyme for melanin synthesis all inhibited OHs-induced hyperactivity. Chemical inhibition and gene knock-down of tyrosine hydroxylase and VMAT2 also induced hyperactivities.
Hyperactivities induced by these treatments were all inhibited by supplementation of L-tyrosine and L-dopa, precursors of dopamine synthesis. Both ncPCB and PBDE decreased dopamine contents and increased the DOPAC/dopamine ratio in whole embryos. The results suggest that functional inhibition of dopaminergic neurons is involved in hyperactivities by OHs. Modulation of swapping of substrate between dopamine synthesis and melanin synthesis could affect neurobehavioral function in development due to incomplete blood brain barrier. Possible delayed effects of OHs in adult stage are also concerned. Our data on cytochrome P450 (CYP) isoform in cat will be also introduced, especially for structure, expression, enzymatic activity and polymorphism.
以上